كلية الطب البيطرى

محتويات مقرر فيرولوجي

Topic

No. of hours

Lecture

Practical

A. Classification of viruses

1

1

 

B. Riboviruses (RNA-viruses):

 

 

 

(1) Orthomyxoviridae

1 ½

3

 

(2) Paramyxoviridae

1 ½

 

 

(3) Coronaviridae

1

1

 

(4) Picornaviridae

1

1

 

(5) Birnaviridae

2

2

 

(6) Reoviridae

 

 

 

(7) Rhabdoviridae

1 ½

1 ½

 

(8) Bunyaviridae

1

1

 

(9) Retroviridae

1

1

 

(10) Flaviviridae

 

 

 

C. Deoxyriboviruses (DNA-viruses):

 

 

 

(1) Poxviridae

1 ½

1 ½

 

(2) Herpesviridae

1 ½

1 ½

 

(3) Adenoviridae

½

½

 

(1) Purification and concentration of viruses

3

 

 

(2) Haemagglutination and haemagglutination inhibition test

6

 

6

(3) Assay of viral infectivity (virus titration)

6

 

6

A. Quantal assay

B. Quantitative assay

(4) Neutralization tests

6

 

6

(5) The immuno fluorescence procedure (fluorescent antibody technique)

3

 

3

(6) Immuno-Enzymatic assay (Enzyme-linked immunosorbent assay)

3

 

3

(7) Immunodiffusion technique (Agar gel precipitation test)

3

 

3

(8) Immuno electrophoresis

2

 

2

(9) Assay of interferon antiviral assay

1

 

1

(10) Molecular virology:

  • Extraction of viral nucleic acid.
  • Hybridization technique.
  • Nucleic acid analysis.
  • PCR and gene cloning
  • Mapping and sequencing

12

 

12

Total

60

15

45

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